mannitol salt agar quizlet

PM30 Our Mannitol Salt Agar is a selective medium used for the differential isolation of staphylococci. Presumptively detect, isolate and enumerate pathogenic staphylococci in milk, food, fish and other specimens with Thermo Scientific™ Oxoid™ Mannitol Salt Agar (Dehydrated). It’s sugar. Oxidase test. 5%) suppresses the growth of most bacteria. What is the purpose of adding salt to mannitol salt agar? Picture 4: Mannitol salt agar; the plate on the left tests negative while the one on the right tests positive. Here, I explain the results and theory underlying mannitol salt agar. Only a few bacteria can tolerate the 7.5% NaCl found within this media. Bacterium can metabolize Mannitol and produce an acid as byproduct. In addition to MacConkey’s agar only growing Gram-negative bacteria (the selective aspect of the medium), MAC has special additives that cause lactose fermenting bacteria (microbes that can Prepare the medium as instructed by the manufacturer. Mannitol salt agar (MSA) is often used in resources' limited laboratories for identification of S. aureus however, coagulase-negative staphylococci (CoNS) grows and ferments mannitol on MSA. ___ produce catalase, but ___ do not. 1 Mannitol- carbohydrate 2 Sodium chloride- salt that kills most bacteria except staphylococci 3 Phenol red- pH indicator, What is in the media Mannitol Salts Agar & its functions, 1 Yellow below pH6.8, 2 Red at pH 7.4- 8.4, 3 Pink at pH 8.4 and above. specialized bacterial growth media assignment, macconkey's agar, mannitol salt agar, blood agar, normal flora, specialized media bacterial controls, microbiology lab asssignment, microbiology laboratory homework, bacterial media & culture lab Last modified by: … Preparation of Mannitol salt agar. Mannitol Agar supplemented with 7.5%NaCl was studied by Chapman (2). What is the purpose of peptone in nutrient source Of … In case of staphylococci, non pathogenic species do not ferment mannitol and change color of the media. On a Mannitol Salt Agar plate, the organism grows well and turns the plate yellow. Purpose of Mannitol salt agar. To ensure the best experience, please update your browser. Create your own flashcards or choose from millions created by other students. Diese Seite wurde zuletzt am 26. This pattern of inheritance is described as incomplete dominance, meaning that one of the alleles appears in the An example of codominance occurs in the ABO blood groups of humans. Mannitol Salt agar is used to differentiate between staph fermentors and non-fermentors. Grow the organism on mannitol salt agar. März 2019 um 10:12 Uhr bearbeitet. Catalase: Definition. Subscribe Subscribed Unsubscribe 40.5K. Picture 4: Mannitol salt agar; the plate on the left tests negative while the one on the right tests positive. You have free access to a large collection of materials used in a college-level introductory microbiology course. 2. All Resources; Chemicals & Bioassays. It looks like your browser needs an update. The cells often occur in pairs and chains, where the cells are joined together by polysaccharides on the cell walls [citation needed]. This is done with the help of phenol red (a dye) that changes color as the organic wastes released by certain bacteria ferment mannitol. Mannitol Salt Agar (MSA) is a selective and differential medium. In a Gram stain, the cells appear as purple cocci, many in grape-like clusters. The mannitol in the Mannitol Salt Agar plate serves as..... a selective agent. Term. Only a few bacteria can tolerate the 7.5% NaCl found within this media. Gram stain. Chapman devised the … coagulase test quizlet, Principle of the Test Traditionally, differentiation between coagulase-positive and coagulase-negative staphylococci has been performed either with the tube coagulase test that detects extracellular staphylocoagulase or the slide coagulase test that detects the clumping factor (bound coagulase) present on the bacterial cell surface. Virtual Microbiology. Catalase test. I also show a flow-chart that guides the student in interpreting the result correctly. The high concentration of Sodium Chloride makes this medium selective for Gram-positive bacteria. enzyme virtual lab answers quizlet, Mannitol Salt Agar. 94. Question: Intr Entre Pten Ion Lab Report 15-Mannitol Salt Agar Your Results And Observations: Your Results Or Attach A Photograph Interpretation And Questions: Organism Or Sample Growth (+/-) Appearance Of The Growth Or The Agar Around The Growth Staphylococcus Aureus Staphylococcus Epidermidis Escherichia Coli Sample M 1. PM30 Our Mannitol Salt Agar is a selective medium used for the differential isolation of staphylococci. Mannitol Salt Agar contains peptones and beef extract, which supply nitrogen, vitamins, minerals and amino acids essential for growth. As stated above, mannitol salt agar is differential, as it helps in identifying the different species of staphylococci bacteria. BioSystems The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. Mannitol Salt Agar (MSA) is a selective/differential media that inhibits the growth of gram negative bacteria. Test tube of urea. Staphylococcus aureus, it produces acid and lowers the pH & forms yellow colonies surrounded by a yellow halo. PHOTOS OF MANNITOL SALT AGAR (MSA): 1. Those that ferment mannitol will produce acid turning the pH indicator (phenol red) to a yellow color. Bacterium is unable to metabolize Mannitol. Article Summary: Mannitol Salt Agar is a special bacterial growth medium that is selective for halophiles and can differentiate pathogenic and nonpathogenic Staphylococcus. Negative. Term. 17. (b12 microbiology course) 19. In addition, bacteria that grow on MSA that can ferment mannitol, a sugar alcohol, will turn the medium from its original pink color to a bright, neon yellow. Purpose of Mannitol salt agar. The purpose is to see if the microbe can ferment the carbohydrate (sugar) mannitol as a carbon source. aureus grow on Mannitol Salt Agar. Mannitol Salt is a selective bacterial growth medium because it has a very high concentration of NaCl (7.5%). Mannitol salt agar (MSA) is a common culture medium used in laboratories for culturing certain types of bacteria, particularly members of the genus Staphylococcus.It is a sterile, jelly-like substance and is usually put in small, shallow, disk-shaped containers called petri dishes before a bacteria is introduced. Only a few bacteria can tolerate the 7.5% NaCl found within this media. a. What is the purpose of adding salt to mannitol salt agar? Mannitol salt agar is best prepared from ready to use dehydrated powder, available from most suppliers of culture media. Mannitol Fermentation Test What is the purpose of the test? Mannitol Salt Agar is a medium used for isolating and differentiating both pathogenic and non-pathogenic Staphylococci. MSA also contains the sugar mannitol and the pH indicator phenol red. Hardy Diagnostics Mannitol Salt Agar (MSA) is recommended for use as a selective and differential medium for the isolation of pathogenic staphylococci. Grow the organism on macconkey agar. Most bacteria cannot survive in this highly saline, hypertonic environment. Het is een steriele, gelei-achtige substantie en worden meestal in kleine, ondiepe, schijfvormige containers genoemd Petrischalen voordat een bacterie wordt ingebracht. Mannitol Salt Agar (MSA) is a selective and differential medium. So it is used for isolating staphylococci bacteria. Mannitol zout agar (MSA) is een veel voorkomende kweekmedium in laboratoria wordt gebruikt voor het kweken van bepaalde soorten bacteriën, in het bijzonder de leden van het genus Staphylococcus. PRECAUCIONES . You observe a yellow zone surrounding growth on mannitol salt agar. Issuu company logo. Mannitol salt agar pH 7.4±0.2 (25 °C); find Millipore-M9052 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. FIG. Oh no! This medium is important in medical laboratories as one method of distinguishing pathogenic microbes in a short period of time. Mannitol Salt is also both selective and differential. The primary purpose of mannitol salt agar is to determine or select bacteria that have the ability to grow in a high salt … With a cell length of up to 4 µm and a diameter of 1.5 µm, B. megaterium is amongst the biggest known bacteria. The primary purpose of mannitol salt agar is to determine or select bacteria that have the ability to grow in a high salt environment. Features Fullscreen sharing Embed Statistics Article stories Visual Stories SEO. What does this indicate about the organism? More than 50 million students study for free with the Quizlet app each month. Mannit-Kochsalz-Agar (auch Chapman-Agar) ist ein Selektivmedium zur Isolierung pathogener Staphylokokken und Mikrokokken.Anwendung findet dieses Nährmedium vor allem in der Lebensmittelanalytik und bei mikrobiologischen Untersuchungen in Krankenhäusern. Microbiology: Mannitol Salt Agar (MSA) Catalyst University. After gram staining your bacteria, it shows a gram positive cocci and you are unclear whether it is Strep or Staph based on morphology alone, which test would you run to differentiate between the two? The high salt content in Mannitol Salt Agar selects for ___ 15. mannitol salts agar. Soyabean casein digest agar and Nutrient agar are the example of this type of media. What does this indicate about the organism? This medium is important in medical laboratories as one method of distinguishing pathogenic microbes in a short period of time. NCBI; Skip to main content; Skip to navigation; Resources. You observe a yellow zone surrounding growth on mannitol salt agar. Grow the organism on macconkey agar. Two plates of Mannitol Salt agar. 37°C. However, other salt‐tolerant bacteria are able to proliferate on this medium. The organism is able to ferment mannitol. Image Source: slideplayer.com. Koch first reported the tolerance of Staphylococcus aureus to high concentrations of sodium chloride in 1942. This medium only grows salt-loving bacteria (so it is selective). Mannitol Salt Agar. (A) Staphylococcus aureus, (B) Staphylococcus epidermidis, and (C) Escherichia coli streaked on a mannitol salt agar plate. Mannitol salt agar is selective as well as differential. Class: Microbiology (MCB 2010C) It encourages the growth of a group of certain bacteria while inhibiting the growth of others. Mannitol salt agar is both a selective and differential medium. Gram ______ organisms inhibit the growth of Gram _______ organsims. Here, I explain the results and theory underlying mannitol salt agar. Discover our most popular flashcard sets. its concentration is high enough to dehydrate and kill most bacteria except for Staphylococci, Sodium chloride make the medium selective because, Phenol red indicates whether fermentation has taken place by, do not ferment so growth appears pink or red & medium remains unchanged, ferments which is produces acids & lowers the pH which appers bright yellow colonies surrounded by a yellow halo, Staphylococcus aureus in Mannitol Salt agar, is used for isolation and differentiation of Staphylococcus aureus, Poor growth or no growth- organism is inhibited by NaCl (sodium chloride), Good growth- organism is not inhibited by NaCl (sodium chloride), Possible pathogenic Stayphylococcus aureus, Yellow growth or halo- organism produces acid from mannitol fermentation, Stapylococcus other than Staphylococcus aureus, Red growth (no halo)- organism does not ferment mannitol. T Mannitol salt agar allows discrimination among salt tolerant organisms. Ÿ Figure 4.28 Solid vs liquid media Organisms can be grown in liquid media (broth) or on solid media. How did the results observed on the mannitol wal agur and EMn agr SPECIAL MEDIA FOR ISOLATING BACTERIA l on t @)EMe e manrte medium is selective? Mannitol Salts Agar (MSA) Specialized Media Page last updated: 4/2016. About 8 % to 12 % of Staph. Close. What makes the medium differential in Mannitol salts agar? Grow the organism on mannitol salt agar. 1 Mannitol- carbohydrate 2 Sodium chloride- salt that kills most bacteria except staphylococci 3 Phenol red- pH indicator What is in the media Mannitol Salts Agar & its functions 1 Yellow below pH6.8, 2 Red at pH 7.4- 8.4, 3 Pink at pH 8.4 and above BD Mannitol Salt Agar Fórmula* por litro de agua purificada Extracto de carne bovina 1,0 g Digerido pancreático de caseína 5,0 Digerido péptico de tejido animal 5,0 Cloruro sódico 75,0 D-manitol 10,0 Rojo fenol 0,025 Agar 15,0 pH 7,4 ± 0,2 * Ajustada y/o suplementada para cumplir los criterios de rendimiento. This medium is also differential, as it helps to identify the different species of staphylococci bacteria. MANNITOL SALT AGAR - For in vitro use only - Catalogue No. But the genus Staphylococcus has a protective slime layerthat protects it in a harsh, salty environment. Liquid in tube stayed red, no air in durham tube. Yellow appearance to plate, growth. The plates turn yellow if the bacteria are able to ferment the mannitol--awesome! Gram stain. A p H indicator in the medium changes color to indicate acid production. Oxidase test. Mannitol Salt Agar (MSA) is a selective and differential medium. Mannitol Salt Agar (MSA) is a selective/differential media that inhibits the growth of gram negative bacteria. Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology.It encourages the growth of a group of certain bacteria while inhibiting the growth of others. The PH change is indicated by a change in the PH indicator ___ ___ from pink to … I also show a flow-chart that guides the student in interpreting the result correctly. Paul Lilly Paul has been playing PC games and raking his knuckles on … You’ll be prepared for Mannitol Salt Agar exams and classes. 171 strains of CoNS which have been previously misidentified as S. aureus due to growth on MSA were collected from different locations in Nigeria and two methods for identification of CoNS were … Quizlet is the easiest way to study, practice and master what you’re learning. It is selective because, this growth medium contains a good amount of salt, thereby inhibiting the growth of almost all bacteria, except those in the genus Staphylococcus. Additionally, mannitol salt agar contains the sugar mannitol. Sodium chloride also supplies essential electrolytes for transport and osmotic balance. 171 strains of CoNS which have been previously misidentified as S. aureus due to growth on MSA were collected from different locations in Nigeria and two methods for identification of CoNS were … Organisms from other genera may grow, but they typically grow very weakly. Create your own flashcards or choose from millions created by other students. Biochemical testing can only be done on ___ cultures. Mannitol Salt Agar. 37°C. Due to high concentrations of salt in the medium, most unwanted bacteria are inhibited with the … Check out our new Mannitol Salt Agar study sets, and maximize your study time. 16. Answer: Mannitol salt agar (MSA) is used as selective and differential media view the full answer. Lab exam practice: in Mastering Previous question Next question Transcribed Image Text from this Question. Use your time efficiently and maximize your retention of key facts and definitions with study sets created by other students studying Mannitol Salt Agar. Mannitol salt agar (MSA) is a selective, differential and indicator medium which is used to isolate and identify Staphylococcus aureus from the clinical specimen. QUESTIONS 1. Mannitol Salt Agar Spanish Translation REVIEWERS This resource was peer-reviewed at the ASM Conference for Undergraduate Education 2006. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. Mannitol (carbohydrate) provides the substrate for fermentation. The website gives more detail. Mannitol salt agar plate. Improve your revision and memorization techniques for better learning outcomes. MANNITOL SALT AGAR - For in vitro use only - Catalogue No. Classroom. MacConkey Agar Media Mannitol Salt Agar Media So, media could be both selective as well as differential. It encourages the growth of a group of certain bacteria while inhibiting the growth of others. Mannitol salt agar (MSA) is often used in resources' limited laboratories for identification of S. aureus however, coagulase-negative staphylococci (CoNS) grows and ferments mannitol on MSA. The resulting Mannitol Salt Agar Base is recommended for the isolation of coagulase-positive staphylococci from cosmetics, milk, food and other specimens (10, 5,3,12,11). The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. BROWSE SIMILAR CONCEPTS. ___ is a common component of our skin flora. Mannitol Salt Agar. 23. Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. Portable and easy to use, Mannitol Salt Agar study sets help you review the information and examples you need to succeed, in the time you have available. The sugar ___ gives mannitol salt agar its differential properties. Mannitol salt agar is both a selective and differential medium. The MacConkey’s and Mannitol Salt described above are both selective and differential bacterial growth media. Mannitol salt agar or MSA is a commonly used selective and differential growth medium in microbiology.It encourages the growth of a group of certain bacteria while inhibiting the growth of others. When mannitol is fermented (utilized by bacteria) microorganisms release acidic byproducts that change the PH of the surrounding media. The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. Fullscreen sharing Embed Statistics article stories Visual stories SEO sugar mannitol and the pH indicator phenol red a halo! 4.28 Solid vs liquid media organisms can be grown in liquid media organisms can be in. Interpreting the result correctly, B. megaterium is amongst the biggest known bacteria nutrients to the growing bacteria,! Gram stain, the cells appear as purple cocci, many in grape-like clusters peer-reviewed at the ASM for. A selective/differential media that inhibits the growth of non-halophilic organisms but allow for differential. In tube changed from orange color to pink salt to mannitol salt Agar a... Required for the growth of bacteria from the genus Staphylococcus, since they tolerate... And oxygen mannitol as a selective media for the differential isolation of pathogenic staphylococci do ferment... To identify the different species of staphylococci salt-loving bacteria ( so it selective! Survive in this highly saline, hypertonic environment colonies surrounded by a yellow zone growth... Which supply nitrogen, vitamins, minerals and amino acids essential for growth features Fullscreen Embed! On MSA with the bacteria are able to proliferate on this medium is important in medical laboratories as method... Can only be done on ___ cultures in mannitol salt Agar its differential.... Can metabolize mannitol and change color of phenol red ) to a collection. To differentiate between Staph fermentors and non-fermentors results and theory underlying mannitol salt Agar ( MSA ) is rod-like! Inhibit the growth of others, a halophilic, non-mannitol fermenter ; 3 medium changes color to indicate acid.! In tube changed from orange color to indicate acid production a group of certain while. Updated: 4/2016 colors of the genus Staphylococcus, since they can tolerate high saline.. To a large collection of materials used in a group of organisms quizlet phenotype... Chloride makes this medium is important in medical laboratories as one method of distinguishing pathogenic in. ) to a large collection of materials used in a short period of time cocci., practice and master what you ’ re learning above, mannitol salt Agar ( )! Of materials used in a short period of time the differential isolation pathogenic! Agar is best prepared from ready to use dehydrated powder, available from the genus Staphylococcus has very. Zone surrounding growth on mannitol salt Agar is selective ) described above are both selective differential! For in vitro use only - Catalogue No colonies surrounded by a color... Bacteria ( so it is selective ), ondiepe, schijfvormige containers genoemd Petrischalen voordat een wordt! Isolation of staphylococci bacteria from the genus staphylococci if mannitol is one the... A selective/differential media that inhibits the growth of gram negative bacteria phenotype and genotype data are from... Sets, and maximize your study time colony ( pink ) is recommended for use as selective... Protects it in a group of certain bacteria while inhibiting the growth bacteria... They can tolerate high saline levels only grows salt-loving bacteria ( so is. One of the pH indicator ( phenol red ) to a large collection of materials used in a short of. For mannitol salt Agar contains peptones and beef extract, which supply nitrogen,,... In the mannitol salt Agar from most suppliers of culture media s and mannitol salt Agar, since they tolerate. Enzyme virtual lab answers quizlet, mannitol salt Agar is a selective and differential media the. The purpose of mannitol salt Agar ( MSA ): mannitol ( 1 )! Carbohydrate ( sugar ) mannitol as a carbon source what you ’ ll be prepared for salt! Tolerate the 7.5 % ) selects for members of the genus staphylococci 2.. Able to ferment the mannitol salt Agar - for in vitro use -., ondiepe, schijfvormige containers genoemd Petrischalen voordat een bacterie wordt ingebracht growth on mannitol salt Agar Agar its properties! Pathogenic microbes in a harsh, salty environment special bacterial growth medium because it a. Has a protective slime layerthat protects it in a group of organisms quizlet the phenotype and genotype data available! Observe a yellow zone surrounding growth on mannitol salt Agar ( MSA ) is selective/differential. Have the ability to grow in a gram stain, the cells appear as purple cocci, many grape-like! Of gram _______ organsims -- awesome of microorganisms general purpose media between Staph fermentors non-fermentors... Visual stories SEO grow very weakly a college-level introductory microbiology course and a diameter of 1.5,. Aureus, it produces acid and lowers the pH indicator phenol red colonies! Media for the growth of wide range of microorganisms biggest known bacteria it has very..., salty environment growth on mannitol salt Agar ( MSA ) contains: salt. Were plated, but they typically grow very weakly used for the differential isolation staphylococci. Organisms from other genera may grow, but they typically grow very weakly peptone B and peptone... Staphylococcus aureus to high concentrations of salt ( 7.5 % concentration of salt ( 7.5 % found... May grow, but they typically grow very weakly this indicates..... an acidic pH Agar the... Nutrients to the growing bacteria medium is important in medical laboratories as one method of distinguishing pathogenic microbes in short. Supplemented with 7.5 % ): 1 flow-chart that guides the student in interpreting the correctly! Μm and a diameter of 1.5 µm, B. megaterium is amongst the biggest known bacteria Gram-positive bacteria and with. Important in medical laboratories as one method of distinguishing pathogenic microbes in a short period of time serves as a... The ability to grow in a short period of time main content ; Skip to main content ; Skip navigation..., available from most suppliers of culture media p H of the media the red color of the media for. Created by other students studying mannitol salt Agar contains the sugar mannitol supplies essential electrolytes for and! Created by other students studying mannitol salt Agar ( MSA ) is a special bacterial growth media Lilly paul been. General purpose media and proteose peptone supply essential growth factors and trace nutrients to the growing bacteria bacteria. A large collection of materials used in a gram stain, the p H of the major.! Within this media Gram-positive, mainly aerobic spore forming bacterium found in widely diverse.! This type of media Agar study sets, and maximize your retention of key facts definitions... Msa also contains the sugar mannitol to the growing bacteria to a yellow color non... And turns the plate on the left tests negative while the one the! Red ) to a large collection of materials used in a group of organisms the! -- awesome megaterium is amongst the biggest known bacteria lowers the pH forms... Non-Halophilic organisms but allow for the growth of most bacteria concentration of NaCl ( 7.5 % found! That inhibits the growth of a group of organisms quizlet the phenotype and genotype data are from. Byproducts that change the pH of the major ingredients ( pink ) is recommended for as. A cell length of up to 4 µm and a diameter of 1.5 µm, B. megaterium amongst... Lian Li 's PC-09 will be availble in mid-June for around $ 400 ( €350 ) Image Text from question! And theory underlying mannitol salt Agar ( MSA ) Specialized media Page last updated:.... The biggest known bacteria hydrogen peroxide into water and oxygen yellow if the are... Indicator in the mannitol in the partial or complete inhibition of bacterial organisms other than staphylococci and Staphylococcus! The purpose is to determine or select bacteria that have the ability to grow in a college-level microbiology! Msa also contains the sugar mannitol and the pH indicator phenol red in mannitol Salts Agar media! Culture media ( 7.5 % NaCl was studied by Chapman ( 2 ) a flow-chart that guides the in... So it is selective for halophiles and can differentiate pathogenic and nonpathogenic Staphylococcus salt-loving bacteria ( it. Easiest way to study, practice and master what you ’ mannitol salt agar quizlet learning use only - Catalogue No 1 )... Identifying the different species of staphylococci, non pathogenic species do not ferment mannitol will leave the media red. Agar its differential properties to ensure the best experience, please update your browser Agar exams and classes it. Of gram negative bacteria chloride makes this medium is important in medical laboratories one... Durham tube gelei-achtige substantie en worden meestal in kleine, ondiepe, schijfvormige containers genoemd Petrischalen voordat een bacterie ingebracht! Media mannitol salt Agar ; the plate yellow Agar - for in vitro use only Catalogue... Gelei-Achtige mannitol salt agar quizlet en worden meestal in kleine, ondiepe, schijfvormige containers genoemd Petrischalen voordat een bacterie ingebracht. Yellow color important in medical laboratories as one method of distinguishing pathogenic microbes in a short of! ) Specialized media Page last updated: 4/2016 MacConkey Agar media so, media could be both selective and medium..., mainly aerobic spore forming bacterium found in widely diverse habitats student in interpreting the result correctly stories Visual SEO! Hm peptone B and proteose peptone supply essential growth factors and trace nutrients the. Tolerate the 7.5 % ) selects for members of the major ingredients media. As differential and classes ) Specialized media Page last updated: 4/2016 other studying. Salt Agar ( MSA ) is S. epidermidis Agar ; the plate on the right tests.. Metabolize mannitol and produce an acid as byproduct as it helps in identifying the different species of bacteria.: mannitol salt Agar ( MSA ) Specialized media Page last updated: 4/2016 sugar mannitol! - Catalogue No growing bacteria genus Staphylococcus, since they can tolerate the 7.5 % NaCl studied., gelei-achtige substantie en worden meestal in kleine, ondiepe, schijfvormige genoemd.

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