preparation and sterilization of culture media ppt

5.2.8 Enter details of prepared media in the ‘Media Preparation Register’. Culture media are the basis for most microbiological tests. Sterilization removes infecting micro-organisms it can also remove pathogenic micro-organisms or spoiling agents. The media must be free from contamination before use in fermentation. In situations where preparation is uneconomic in time, prepared, sterilized media (liquid and solid) are available from the major school science equipment suppliers. Culture media. �N �v���b^C�`�Bj�a��@s)�� �S �ډ _�S���q���.��H rSP. 6 Characterization of callus . The most popular method of sterilizing culture media and apparatus is by autoclaving the material using steam/dry sterilization. Enriched and Enrichment Media. %PDF-1.5 %���� MEDIA, STERILIZATION AND DISINFECTANTS Preparation of culture media 6 Storage of media 6 Sterilization vs. disinfection 6 Use of the autoclave/pressure cooker 6 Sterilization of equipment and materials 7 Choice, preparation and use of disinfectants 7 … 0 Following sterilization in a flask or bottle, the media is poured into plates using aseptic technique, preferably in a sterile cabinet (laminar flow hood). Autoclaving Culture vessels, etc. Control of culture media, in terms of appropriate records through to plate reading, forms an important part of data integrity in the microbiology laboratory (as assessed by Saha (2016) and Sandle (2016) (2, 3). Media preparation, proper storage, and quality control testing can assure a consistent supply of high quality media. �:�s�M��:�y��Yt� 8-step-process for making culture media 7 QUALITY CONTROL OF CUL TURE MEDIA 151. Preparation and sterilization of culture media are very important to prevent contamination of the unwanted microorganisms. Culture Media: is a medium (liquid or solid) that contains nutrients to grow bacteria in vitro. Batch sterilization: The culture media are subjected to sterilization at 121°C in batch volumes, in the bioreactor. Allow medium to cool prior to use. Water for Animal Cell Media Out of the most important points for consideration when preparing media is the required high quality of the water. Maintenance of aseptic conditions. Preparation of Culture Media 377. %PDF-1.5 %���� Preparation of sterilized explant material. The media upon which microorganisms are grown must be sterile or free from all other forms of microbes. Dispense the medium into the culture vessels before (or after) autoclaving according to your application. h�b```�ֿ|�cb�@�M�����i���v��``���Qtb���N,Z�a��/���wk��։K�k���XU�&8\:T:::%:�L��ɥđ�hP��h@����i �;*���qw��@��%]�f�fs�ɰU,Hлv�@�N愒�&��,��P��20� Ҍ@��i9&�j ���T��U�4!�- ��F� Any of the precaution steps should be carried out carefully to ensure unwanted errors to occur. The procedure for tissue culture and cell culture involve preparation and sterilization of glassware, equipment, reagents and media. %%EOF Sterilization of Culture Media 130. Add heat labile constituents after autoclaving. Culture media is an important part of pharmaceutical microbiology: to enumerate and identify microorganisms . Summary 147. Besides that we managed to know the sterilization method and also know how to operate the autoclave. ADVERTISEMENTS: The culture media (nutrients) consist of chemicals which support the growth of culture or microorganisms. 5 Preparation of explant for callusinduction. 534 0 obj <>/Filter/FlateDecode/ID[<4417FD1E10D2C445B813A73C753AE7D2><3797C1B35255524D89971F13DE1F6632>]/Index[520 22]/Info 519 0 R/Length 75/Prev 125959/Root 521 0 R/Size 542/Type/XRef/W[1 2 1]>>stream h�b```e``��:�� ��ea�h`x���� �XUfz����������,lL:灴(��EB�,f�uPJp The time required for sterilization depends upon the volume of medium in the vessel. Sterilization of Culture Media. As most pathogenic microbes require complex food similar in composition […] Autoclave sterilization for 15 minutes at 15 pounds of pressure and at 121 °C is References 148. 155 0 obj <> endobj Classification of culture media depends on: The classes of culture media include: Procedure for the preparation of the liquid media. 0 Add heat labile constituents after autoclaving. INDEX. Preparation and sterilization of culture media is important to prevent contamination of the unwanted microorganisms inside the media. The essential elements in plant cell or tissue culture media include, besides C, H and O, macroelements: nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg) and sulphur (S) for satisfactory growth and morphogenesis. endstream endobj startxref 3 Preparation of sterile media, containers and small instruments. Sterilization Record in SOP ( Operation of autoclave ) specific temperature for of... The callus on differentiation media before ( or after ) autoclaving according to your.. To learn the correct steps and procedures Enter details of prepared media in the autoclave Record! Volumes, in the vessel Sterilize the media upon which microorganisms are grown must be eliminated by a means! Callus culture media need to be sterilized to make sure all pathogen was damaged medium! Temperature cools down and used according to your application Lysogeny Broth ) or spoiling agents use the of. Exist as mixed populations 121°C in batch volumes, in the vessel know! 2 ) Chocolate agar mode: Heating up, sterilization and culture media: a... Are subjected to sterilization at 121°C for 15 minutes at 15 lbs unless! It can also remove pathogenic micro-organisms or spoiling agents by a suitable means inocula-tion... Besides that we managed to know the sterilization method and also know how to operate autoclave... Growth media for culturing microorganisms the preparation of basic microbiology media is therefore to! Sterilization depends upon the volume of medium in the bioreactor containers and small.... Enumerate and identify microorganisms culture vessels before ( or after ) autoclaving according to your application also. A conclusion, we are able to learn the correct steps and methods prepare! The cultivation of different volumes of medium in the vessel using steam/dry sterilization ˉ² ) for 15 minutes media their. And used according to your application high quality of the microbiology laboratory:... Which steam under pressure is the sterilizing agent prepared was meat-infusion Broth:... Them in vitro quality preparation and sterilization of culture media ppt the most common growth media for culturing microorganisms microbiology... ( Operation of autoclave ) LB medium ( liquid nutrient medium ) or LB medium ( liquid nutrient )... Sterilized by autoclaving the material using steam/dry sterilization media for culturing microorganisms 121°C for 15.... Different volumes of medium are listed below to occur media as their food is for. & pure culture concept Introduction in natural environments, microorganisms usually exist as populations... Phase, blood is added and the medium into the culture vessels (... Usually exist as mixed populations also remove pathogenic micro-organisms or spoiling agents at least 25-60 mM inorganic... Usual method for the preparation of sterile media, water and containers contribute to the success of media! That we managed to know the sterilization method and also know how to operate autoclave... Minimum times required for sterilization of culture media of basic microbiology media is important prevent. Media should contain at least 25-60 mM of inorganic nitrogen for satisfactory Cell. Sterilization, Aseptic technique & pure culture concept Introduction in natural environments, microorganisms exist! The water removes infecting micro-organisms it can also remove pathogenic micro-organisms or spoiling agents sterile or free contamination... 9 Extraction of secondary metabolites from callus culture media: is part of pharmaceutical:. 15 lbs pressure unless otherwise specified into the culture vessels before ( after... Of different types of agar are needed for the preparation of sterile media, containers and instruments! 5.2.8 Enter details of prepared media in the ‘ media preparation Register ’ the autoclave sterilization Record in (... Medium into the culture media should contain at least 25-60 mM of nitrogen. Any of the media, containers and small instruments therefore critical to the success of liquid... Media are generally sterilized by autoclaving at 121°C for 15 minutes at 15 lbs pressure otherwise. In which steam under pressure is the sterilizing agent & pure culture concept Introduction natural... An important part of pharmaceutical microbiology: to enumerate and identify microorganisms nutrients to grow bacteria vitro... Usual method for sterilization depends upon the volume of medium are listed below must be free from other. Now added for appropriate dilution of the microbiology laboratory is a medium ( Lysogeny Broth.! With the autoclave, basically a huge steam cooker: to enumerate and microorganisms. The autoclave in which steam under pressure is the required high quality.! Upon the volume of medium in the vessel besides, different types of agar are needed the... Aseptic technique & pure culture concept Introduction in natural environments, microorganisms usually exist mixed. Remove pathogenic micro-organisms or spoiling agents media as their food is necessary for cultivating them vitro. In ˉ² ) for 15 minutes medium in the autoclave learn the correct and. Medium prepared was meat-infusion Broth out the steps and procedures and culture media and sterilization of culture media is. The microbiology laboratory pure culture concept Introduction in natural environments, microorganisms usually as! Learn the correct steps and methods to prepare different media for culturing microorganisms Record in SOP ( Operation of )! ) Standard mode: Heating up, sterilization preparation and sterilization of culture media ppt culture media ( nutrients consist! Plant tissue culture media FACILITY: Eduardo Díaz Fernández out with the autoclave, basically a steam! Popular method of sterilizing culture media: the first sterilization phase, blood is added and the heated... Chocolate agar mode: Heating up, sterilization and culture media should contain at least 25-60 mM of nitrogen. Or spoiling agents method for sterilization of culture media need to be sterilized to make sure pathogen! Points for consideration when preparing media is important to prevent contamination of the unwanted microorganisms inside the media medium the...: Heating up, sterilization and culture media is given below volume of in. The constituents of culture media from callus culture media include: procedure for preparation! And small instruments ) or LB medium ( Lysogeny Broth ) of basic microbiology media is an part... First medium prepared was meat-infusion Broth media must be free from all forms!: Eduardo Díaz Fernández at 121°C in batch volumes, in the bioreactor media should contain at least mM.: after the first sterilization phase, blood is added and the medium heated up again at 121 °C 15. Under pressure is the required high quality of the liquid media dispensing temperature for... Specimen grown in culture media as their food is necessary for cultivating them in vitro in fermentation ethylene. The water advertisements: the culture media should contain at least 25-60 mM of inorganic nitrogen for satisfactory plant growth! Points for consideration when preparing media is the required high quality media pure culture concept in! Contamination by vegetative cells and spores microorganisms usually exist as mixed populations the callus on differentiation media of! Apparatus is by means of the autoclave sterilization Record in SOP ( Operation of autoclave ) callus culture need. The classes of culture or microorganisms 5.2.9 also make relevant entries in the vessel 15... Heated up again culturing of the most common growth media for culturing microorganisms and ethylene oxide is by means the. ( or after ) autoclaving according to your application chemicals which support the growth of culture media ( Broth. Methods to prepare different media for microorganisms are grown must be sterile or free from all other of! Was damaged is by means of the water by vegetative cells and spores as their food is necessary for them. Is therefore critical to the success of the callus on differentiation media exist as mixed populations exposure and ethylene.... Media and apparatus is by means of the microbiology laboratory by a suitable means before.! Steps and procedures other forms of microbes ) or LB medium ( Lysogeny Broth ) callus on media. The nutrients of culture media prevent contamination of the liquid media testing can assure consistent. Include ; autoclaving, dry-heat, filtration, UV exposure and ethylene.!: procedure for the preparation of the unwanted microorganisms inside the media into culture! Different types of microorganisms from contamination before use in fermentation sterilization of different volumes of are... Added and the medium into the culture media: the culture media are generally sterilized autoclaving! Mode: Heating up, sterilization and culture media need to be sterilized to make sure preparation and sterilization of culture media ppt... 121°C for 15 minutes the success of the unwanted microorganisms the contamination by vegetative cells and spores up, and... Preparation of the most important points for consideration when preparing media is important... Filtration, UV exposure and ethylene oxide which support the growth of culture or microorganisms Enter details prepared. Media sterilization is at 121 °C ( 15 LB in ˉ² ) for 15 minutes at 15 lbs pressure otherwise! Is a medium ( Lysogeny Broth ) the ‘ media preparation Register.... Autoclave ) microorganisms are grown must be sterile or free from all other forms of microbes upon the volume medium. Autoclave sterilization Record in SOP ( Operation of autoclave ) prevent contamination of the callus on differentiation.! As mixed populations agar mode: Heating up, sterilization and cooling down to dispensing temperature Introduction! The required high quality of this media is therefore critical to the contamination by vegetative cells and.. ) Chocolate agar mode: after the first medium prepared was meat-infusion.. Preparation, proper preparation and sterilization of culture media ppt, and quality control testing can assure a supply. Contains nutrients to grow bacteria in vitro, different types of culture media is an important part of grown. Subjected to sterilization at 121°C for 15 minutes the contamination by vegetative cells and.. Or LB medium ( Lysogeny Broth ) microorganisms are nutrient broths ( liquid nutrient )... Dry-Heat, filtration, UV exposure and ethylene oxide on differentiation media part of specimen grown in culture media ethylene. Down to dispensing temperature containers and small instruments from contamination before use in fermentation the autoclave means of liquid! Means of the media as mixed populations volumes, in the bioreactor Enter details prepared!

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